Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment
Identifieur interne : 000090 ( France/Analysis ); précédent : 000089; suivant : 000091Tetraploidization events by chromosome doubling of nucellar cells are frequent in apomictic citrus and are dependent on genotype and environment
Auteurs : Pablo Aleza [Espagne] ; Yann Froelicher [France] ; Sergio Schwarz [Espagne] ; Manuel Agustí [Espagne] ; María Hernández [Espagne] ; José Juárez [Espagne] ; François Luro [France] ; Raphael Morillon [France] ; Luis Navarro [Espagne] ; Patrick Ollitrault [Espagne, France]Source :
- Annals of Botany [ 0305-7364 ] ; 2011.
English descriptors
- KwdEn :
- Alleles, Biological Evolution, Breeding, Chromosomes, Plant (genetics), Citrus (embryology), Citrus (genetics), Cluster Analysis, Diploidy, Environment, Genetic Markers, Genetic Variation (genetics), Genotype, Hybridization, Genetic, Seedlings (genetics), Seeds (embryology), Seeds (genetics), Tetraploidy.
- MESH :
- chemical : Genetic Markers.
- embryology : Citrus, Seeds.
- genetics : Chromosomes, Plant, Citrus, Genetic Variation, Seedlings, Seeds.
- Alleles, Biological Evolution, Breeding, Cluster Analysis, Diploidy, Environment, Genotype, Hybridization, Genetic, Tetraploidy.
Abstract
Polyploidy is a major component of plant evolution. The citrus gene pool is essentially diploid but tetraploid plants are frequently encountered in seedlings of diploid apomictic genotypes. The main objectives of the present study were to establish the origin of these tetraploid plants and to ascertain the importance of genotypic and environmental factors on tetraploid formation.
Tetraploid seedlings from 30 diploid apomictic genotypes were selected by flow cytometry and genotyped with 24 single sequence repeat (SSR) markers to analyse their genetic origin. Embryo rescue was used to grow all embryos contained in polyembryonic seeds of ‘Tardivo di Ciaculli’ mandarin, followed by characterization of the plantlets obtained by flow cytometry and SSR markers to accurately establish the rate of tetraploidization events and their potential tissue location. Inter-annual variations in tetraploid seedling rates were analysed for seven genotypes. Variation in tetraploid plantlet rates was analysed between different seedlings of the same genotype (‘Carrizo’ citrange;
Tetraploid plants were obtained for all the studied diploid genotypes, except for four mandarins. All tetraploid plants were identical to their diploid maternal line for SSR markers and were not cytochimeric. Significant genotypic and environmental effects were observed, as well as negative correlation between mean temperature during the flowering period and tetraploidy seedling rates. The higher frequencies (20 %) of tetraploids were observed for citranges cultivated in the Mediterranean area.
Tetraploidization by chromosome doubling of nucellar cells are frequent events in apomictic citrus, and are affected by both genotypic and environmental factors. Colder conditions in marginal climatic areas appear to favour the expression of tetraploidization. Tetraploid genotypes arising from chromosome doubling of apomictic citrus are extensively being used as parents in breeding programmes to develop seedless triploid cultivars and have potential direct use as new rootstocks.
Url:
DOI: 10.1093/aob/mcr099
PubMed: 21586529
PubMed Central: 3119611
Affiliations:
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PMC:3119611Le document en format XML
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Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name><affiliation wicri:level="1"><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. 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Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Froelicher, Yann" sort="Froelicher, Yann" uniqKey="Froelicher Y" first="Yann" last="Froelicher">Yann Froelicher</name><affiliation wicri:level="3"><nlm:aff id="af2"><addr-line>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region></placeName></affiliation></author><author><name sortKey="Schwarz, Sergio" sort="Schwarz, Sergio" uniqKey="Schwarz S" first="Sergio" last="Schwarz">Sergio Schwarz</name><affiliation wicri:level="1"><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Agusti, Manuel" sort="Agusti, Manuel" uniqKey="Agusti M" first="Manuel" last="Agustí">Manuel Agustí</name><affiliation wicri:level="2"><nlm:aff id="af3"><addr-line>Instituto Agroforestal Mediterráneo, Universidad Politécnica, Camino de Vera, s/n, 46022 Valencia, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto Agroforestal Mediterráneo, Universidad Politécnica, Camino de Vera, s/n, 46022 Valencia</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Hernandez, Maria" sort="Hernandez, Maria" uniqKey="Hernandez M" first="María" last="Hernández">María Hernández</name><affiliation wicri:level="1"><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Juarez, Jose" sort="Juarez, Jose" uniqKey="Juarez J" first="José" last="Juárez">José Juárez</name><affiliation wicri:level="1"><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Luro, Francois" sort="Luro, Francois" uniqKey="Luro F" first="François" last="Luro">François Luro</name><affiliation wicri:level="1"><nlm:aff id="af4"><addr-line>Unité GEQA, INRA, San Giuliano 20230 San Nicolao, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Unité GEQA, INRA, San Giuliano 20230 San Nicolao</wicri:regionArea><wicri:noRegion>20230 San Nicolao</wicri:noRegion><wicri:noRegion>20230 San Nicolao</wicri:noRegion></affiliation></author><author><name sortKey="Morillon, Raphael" sort="Morillon, Raphael" uniqKey="Morillon R" first="Raphael" last="Morillon">Raphael Morillon</name><affiliation wicri:level="3"><nlm:aff id="af2"><addr-line>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region></placeName></affiliation></author><author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name><affiliation wicri:level="1"><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name><affiliation wicri:level="1"><nlm:aff id="af1"><addr-line>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia, Spain</addr-line></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4·5, 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="af2"><addr-line>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region></placeName></affiliation></author></analytic><series><title level="j">Annals of Botany</title><idno type="ISSN">0305-7364</idno><idno type="eISSN">1095-8290</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alleles</term><term>Biological Evolution</term><term>Breeding</term><term>Chromosomes, Plant (genetics)</term><term>Citrus (embryology)</term><term>Citrus (genetics)</term><term>Cluster Analysis</term><term>Diploidy</term><term>Environment</term><term>Genetic Markers</term><term>Genetic Variation (genetics)</term><term>Genotype</term><term>Hybridization, Genetic</term><term>Seedlings (genetics)</term><term>Seeds (embryology)</term><term>Seeds (genetics)</term><term>Tetraploidy</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Genetic Markers</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Citrus</term><term>Seeds</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes, Plant</term><term>Citrus</term><term>Genetic Variation</term><term>Seedlings</term><term>Seeds</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alleles</term><term>Biological Evolution</term><term>Breeding</term><term>Cluster Analysis</term><term>Diploidy</term><term>Environment</term><term>Genotype</term><term>Hybridization, Genetic</term><term>Tetraploidy</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background and Aims</title><p>Polyploidy is a major component of plant evolution. The citrus gene pool is essentially diploid but tetraploid plants are frequently encountered in seedlings of diploid apomictic genotypes. The main objectives of the present study were to establish the origin of these tetraploid plants and to ascertain the importance of genotypic and environmental factors on tetraploid formation.</p></sec><sec><title>Methods</title><p>Tetraploid seedlings from 30 diploid apomictic genotypes were selected by flow cytometry and genotyped with 24 single sequence repeat (SSR) markers to analyse their genetic origin. Embryo rescue was used to grow all embryos contained in polyembryonic seeds of ‘Tardivo di Ciaculli’ mandarin, followed by characterization of the plantlets obtained by flow cytometry and SSR markers to accurately establish the rate of tetraploidization events and their potential tissue location. Inter-annual variations in tetraploid seedling rates were analysed for seven genotypes. Variation in tetraploid plantlet rates was analysed between different seedlings of the same genotype (‘Carrizo’ citrange; <italic>Citrus sinensis</italic> × <italic>Poncirus trifoliata</italic>) from seeds collected in different tropical, subtropical and Mediterranean countries.</p></sec><sec><title>Key Results</title><p>Tetraploid plants were obtained for all the studied diploid genotypes, except for four mandarins. All tetraploid plants were identical to their diploid maternal line for SSR markers and were not cytochimeric. Significant genotypic and environmental effects were observed, as well as negative correlation between mean temperature during the flowering period and tetraploidy seedling rates. The higher frequencies (20 %) of tetraploids were observed for citranges cultivated in the Mediterranean area.</p></sec><sec><title>Conclusions</title><p>Tetraploidization by chromosome doubling of nucellar cells are frequent events in apomictic citrus, and are affected by both genotypic and environmental factors. Colder conditions in marginal climatic areas appear to favour the expression of tetraploidization. Tetraploid genotypes arising from chromosome doubling of apomictic citrus are extensively being used as parents in breeding programmes to develop seedless triploid cultivars and have potential direct use as new rootstocks.</p></sec></div></front></TEI><affiliations><list><country><li>Espagne</li><li>France</li></country><region><li>Communauté valencienne</li><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region></list><tree><country name="Espagne"><noRegion><name sortKey="Aleza, Pablo" sort="Aleza, Pablo" uniqKey="Aleza P" first="Pablo" last="Aleza">Pablo Aleza</name></noRegion><name sortKey="Agusti, Manuel" sort="Agusti, Manuel" uniqKey="Agusti M" first="Manuel" last="Agustí">Manuel Agustí</name><name sortKey="Hernandez, Maria" sort="Hernandez, Maria" uniqKey="Hernandez M" first="María" last="Hernández">María Hernández</name><name sortKey="Juarez, Jose" sort="Juarez, Jose" uniqKey="Juarez J" first="José" last="Juárez">José Juárez</name><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name><name sortKey="Schwarz, Sergio" sort="Schwarz, Sergio" uniqKey="Schwarz S" first="Sergio" last="Schwarz">Sergio Schwarz</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Froelicher, Yann" sort="Froelicher, Yann" uniqKey="Froelicher Y" first="Yann" last="Froelicher">Yann Froelicher</name></region><name sortKey="Luro, Francois" sort="Luro, Francois" uniqKey="Luro F" first="François" last="Luro">François Luro</name><name sortKey="Morillon, Raphael" sort="Morillon, Raphael" uniqKey="Morillon R" first="Raphael" last="Morillon">Raphael Morillon</name><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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